T7 Designer

Based on Python script by @cdcruzher

Template & Primer Generator

Enter your RNA sequence to automatically generate the T7 promoter-fused ssDNA template and optimize PCR primers.

Sequence (5'→3') Supports RNA (U) or DNA (T)

Label (Optional)

Forward Primer Length

Reverse Primer Length

Auto-optimize Tm

Adjusts Reverse length to match Forward Tm.

Label

Input Sanitized

T7 Promoter

Start G

RNA Seq

Show separate Anti-Sense strand (5'→3')

Tm: °C

Tm: °C

Forward Primer

Reverse Primer