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AuNP MasterCalc

Created by Carlos David @cdcruzher

1. Particle Physics

nm
bases
Viz: Coverage
Surface Area -- nm²
Theoretical Max Load -- strands
Loading Density --

2. Lab Recipe Calculator

Experiment Prep

Your Sample

µL

≈ 6.02 × 10¹² nps/mL

100% (Monolayer)
10% 100% Excess (1000%)
µM

Required Reagents

Add this amount of Oligo Stock:

-- µL
Total Oligo Amount -- pmol
Final Oligo Conc. -- µM
Oligo : Au Ratio -- : 1
To achieve 100% coverage on 13nm AuNPs.

Calculation Logic

  • Step 1: Max Loading (N) derived from Surface Area & length-dependent density (approx 0.3-0.1 strands/nm²).
  • Step 2: Total AuNPs = (Concentration × Volume).
  • Step 3: Required Oligo Moles = (Total AuNPs × N × Coverage%).
  • Step 4: Volume to Add = Required Moles / Stock Concentration.

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